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Analytical Chemistry & Spectroscopy

Mobile Phase

The fluid that carries the sample through the stationary phase during chromatography.

Unit mL/min

In depth

Also called the eluent or carrier, the mobile phase competes with the stationary phase for the analytes, so changing its composition changes the separation. Gradient elution steadily alters that composition to separate compounds of widely different retention in one run.

Examples in the real world

Helium carrier gas in GC; a water and acetonitrile gradient in HPLC.