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Analytical Chemistry & Spectroscopy

Isotopic Peak

A peak appearing at a higher mass than the main ion because the molecule contains a heavier isotope.

Formula / equation M+1, M+2
Unit Da

In depth

Their relative heights reveal the elements present: chlorine gives an M+2 peak one third the height of M, bromine gives one of equal height, and the M+1 peak from carbon-13 grows by about 1.1% for each carbon atom.

Examples in the real world

A 3:1 pair of peaks two mass units apart immediately identifying a chlorine-containing compound.